Tbk1 Knockout cell line(RAW 264.7)

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location： Home > Products > KO Cell Lines > Tbk1 Knockout cell line(RAW 264.7)

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Tbk1 Knockout cell line(RAW 264.7) Order

Tbk1 Knockout cell line(RAW 264.7)
Catalog#
YKO-M140
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Tbk1 Knockout cell line(RAW 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Tbk1 Knockout cell line (RAW 264.7)
Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Mouse
Gene Name
Tbk1
Gene ID
56480
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Tbk1-201)
Validation: PCR+Sanger Sequencing
Tbk1 Knockout cell line(RAW 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tbk1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tbk1 gene knockout Tbk1 Knockout cell line (RAW 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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